Partners: University of Dundee

Staff: Prof. Angus Lamond FRS, Dr. Armel Nicolas

The Lamond group have a long standing interest in using quantitative methods to study nuclear structure and gene expression, including mass spectrometry based proteomics and live cell fluorescence imaging. Together with their collaborators the Lamond group have made major contributions to developing next generation quantitative proteomics methods which they have used in system-wide studies in mammalian cells and model organisms of ‘protein properties’ (Lamond et. al., 2012). This includes the measurement of subcellular protein localisation, protein abundance, protein-protein interactions and rates of protein synthesis, degradation and turnover, which are related to patterns of post translational protein modification and isoform expression.

The Lamond group are also developing new software and computational approaches for the management, analysis and visualisation of proteomics ‘Big Data’ (see www.PepTracker.com). The group have created ‘The Encyclopedia of Proteome Dynamics’, an online database with viewers allowing them to share large-scale, complex proteomics datasets with the life sciences community. The expertise of the Lamond group in MS-based proteomics and data analysis will be used in this project to perform detailed analyses of the effects of FMDV infection on host cell proteomes.

Reference:

Advancing cell biology through proteomics in space and time (PROSPECTS). Lamond AI, Uhlen M, Horning S, Makarov A, Robinson CV, Serrano L, Hartl FU, Baumeister W, Werenskiold AK, Andersen JS, Vorm O, Linial M, Aebersold R, Mann M. Mol Cell Proteomics. 2012 Mar;11(3):O112.017731. Epub 2012 Feb 6.